RBA細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
器官來源： 乳腺
ATCC Number： CRL-1747?
相關(guān)**： 腺癌
數(shù)量： 大量
品系： Sprague-Dawley
細(xì)胞類型： 其他細(xì)胞類型
年限： 50 days *****
是否是腫瘤細(xì)胞： 0
RBA細(xì)胞物種來源： 褐鼠
Designations: RBA
Depositors: LA Cohen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus
Morphology: epithelial
Source: Organ: mammary gland
Strain: Sprague-Dawley
Disease: adenocarcinoma
Cell Type: chemically induced
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.RBA細(xì)胞 Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: androgen receptor
glucocorticoid
insulin
androgen receptor, positive; glucocorticoid; insulin
Tumorigenic: Yes
Age: 50 days *****
Gender: female
Comments: The cells do not possess significant amounts of estradiol receptors.
The cells contain an activated H-ras (ras) oncogene.
The line lacks vimentin and reacts strongly with anti keratin antibodies (evidence of epithelial origin).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
RBA細(xì)胞Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Doubling Time: 18 to 20 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22313: Cohen LA, et al. Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture. In Vitro 10: 51-62, 1974. PubMed: 4220124
22669: Cohen LA, Karmali RA. Endogenous prostaglandin production by established cultures of neoplastic rat mammary epithelial cells. In Vitro 20: 119-126, 1984. PubMed: 6423517
22689: Vignon F, et al. RBA細(xì)胞Hormonal regulation in two rat mammary cancer cell lines: glucocorticoid and androgen receptors. Mol. Cell. Endocrinol. 13: 191-202, 1979. PubMed: 109329
