E5BB3IIA2細胞
細胞形態： **樣
是否是腫瘤細胞： 0
物種來源： 小鼠
**類型： IgG2a; kappa light chain
生長狀態： 懸浮生長
運輸方式： 凍存運輸
ATCC Number： HB-121?
數量： 大量
E5BB3IIA2細胞Designations: E5BB3IIA2
Depositors: RP Siraganian
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: E5BB3IIA2細胞immunoglobulin; monoclonal antibody; against human IgE
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with purified IgE myeloma protein.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody induces histamine release from human basophils.
E5BB3IIA2細胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 58009: Hook WA, et al. E5BB3IIA2細胞Detection of different antigenic sites on human IgE using monoclonal antibodies. Fed. Proc. 42: 713, 1983.
