RH/K34細(xì)胞
ATCC Number： CRL-2561?
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 其他
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
器官來源： 外周血
運輸方式： 凍存運輸
數(shù)量： 大量
Designations: RH/K34
Depositors: RM Simpson
RH/K34細(xì)胞Biosafety Level: 2 [Cells infected with HTLV-I virus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates and some adherent cells
Organism: Oryctolagus cuniculus deposited as rabbit
Morphology: lymphoblast
Source: Organ: peripheral blood
Cell Type: lymphocyteinfected with HTLV-I
Cellular Products: HTLV-I (human T cell leukemia virus)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RH/K34細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: 1. This cell line was deposited at the ATCC by Dr. R. Mark Simpson and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of these cells, or their products must first be negotiated with the National Institute of Allergy and Infectious Diseases (NIAID) Office of Technology Development (OTD), Building 31, Room 3B-62 31 Center Drive, MSC 2137 Bethesda, MD 20892-2137 TEL: 301-496-2644 FAX: 301-402-7123
Isolation: Isolation date: 1991
Receptors: T cell antigen receptor gamma (TCR), not expressed; interleukin-2 receptor (IL-2R) + [50673]
Virus Susceptibility: Human immunodeficiency virus 1 (HIV-1) (weakly)
Antigen Expression: CD5 +/-; CD4 -; CD8 -; MHC class II + [50673]
Gender: unknown
Comments: The RH/K34 gamma/delta T cell line was derived from rabbit peripheral blood mononuclear cells (PBMC) by coculture with the rabbit RHT-16 cell line. RH/K34細(xì)胞[50673]
RHT-16 contains integrated HTLV-1 leukemia virus-I (HTLV-1) provirus and sheds intact virus particles.
The RH/K30 (ATCC CRL-2560) and RH/K34 (ATCC CRL-2561) cell lines differ in their potential to cause disease in rabbits.
Injection of the RH/K34 cell line causes human ***** T-cell leukemia/lymphoma-like (ATLL) disease that is fatal within 10 days.
However, all rabbits injected with the same or higher doses of RH/K30 survived with a low-grade leukemia that showed evidence of acute rejection. [50677]
The cell line may be used as an infectious human disease model; it can be used to infect rabbits with HTLV-1.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml. Do not allow the cell concentration to exceed 2 X 10(6) cells/ml.
Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml.
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 50673:RH/K34細(xì)胞 Sawasdikosol S, et al. Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I. J. Exp. Med. 178: 1337-1345, 1993. PubMed: 8376938
50677: Zhao TM, et al. Variation in HTLV-I sequences from rabbit cell lines with diverse in vivo effects. Virology 195: 271-274, 1993. PubMed: 8317104
50751: Mahana W, et al. Genes in the pX region of human T cell leukemia virus I influence Vav phosphorylation in T cells. Proc. Natl. Acad. Sci. USA 95: 1782-1787, 1998. PubMed: 9465094
50752: Simpson RM, et al. Experimental acute ***** T cell leukemia-lymphoma is associated with thymic atrophy in human T cell leukemia virus type I infection. Lab. Invest. 74: 696-710, 1996. PubMed: 8600320
50753: Chouchane L, et al. Heat-shock proteins expressed on the surface of human T cell leukemia virus type I-infected cell lines induce autoantibodies in rabbits. J. Infect. Dis. 169: 253-259, 1994. PubMed: 7508967
