EL-NC-1S細胞
ATCC Number： HB-9647?
運輸方式： 凍存運輸
生長狀態： 懸浮生長
是否是腫瘤細胞： 0
物種來源： 小鼠
數量： 大量
細胞形態： **樣
**類型： IgG1
Designations: EL-NC-1S [anti NAP-1]
Depositors: National Cancer Institute/FCRF
EL-NC-1S細胞Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against neutrophil attractant/activation protein 1 (NAP-1, also known as interleukin 8 or IL-8 and monocyte derived neutrophil chemotactic factor, MDNCF)
Permits/Forms: EL-NC-1S細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF). [22353]
Spleen cells were fused with NS-1 mouse myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: EL-NC-1S細胞Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
References: 22353: Yoshimura T, et al. Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence. Mol. Immunol. 26: 87-93, 1989. PubMed: 2648135
23250: Sylvester I, et al. Secretion of neutrophil attractant/activation protein by lipopolysaccharide-stimulated lung macrophages determined by both enzyme-linked immunosorbent assay and N-terminal sequence analysis. Am. Rev. Respir. Dis. 141: 683-688, 1990. PubMed: 2178529
