34-2-12S細胞
細胞形態： **樣
**類型： IgG2a; kappa light chain
生長狀態： 懸浮生長
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-87?
34-2-12S細胞Designations: 34-2-12S
Depositors: DH Sachs, K Ozato
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 34-2-12S細胞immunoglobulin; monoclonal antibody; against H-2 D d; cytotoxic
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Comments: Animals were immunized with spleen cells from BDF1 mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody specificity is different from those of 34-4-21S (ATCC HB-76) and 34-5-8S (ATCC HB-102).
Tested and found negative for ectromelia virus (mousepox).
Propagation: 34-2-12S細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 58003: Ozato K, et al. 34-2-12S細胞Monoclonal antibodies to mouse MHC antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation 34: 113-118, 1982. PubMed: 7135466
