3A5細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： CRL-2440?
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgM; kappa light chain
生長狀態(tài)： 懸浮生長
Designations: 3A5
Depositors: PJ Bjorkman
3A5細(xì)胞Isotype: IgM; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma:
Cellular Products: immunoglobulin; monoclonal antibody; against HFE
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Comments: 3A5細(xì)胞The cell line was established by Jose A. Lebron and Pamela J. Bjorkman in 1997.
Animals were tolerized with human beta2-microglobulin and immunized with purified, soluble, human HFE.
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
The antibody recognizes the HFE portion of the HFE/beta2 heterodimer.
HFE is a major histocompatibility complex (MHC) related protein that is mutated in the iron-overload disease hereditary hemochromatosis.
This antibody can be used for ELISA applications and to immunoprecipitate soluble HFE; it is not reactive in Western Blot assays.
Propagation: ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
Temperature: 37.0°C
Subculturing: 3A5細(xì)胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp4 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml.
Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.
Preservation: HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%.
Related Products: recommended serum:ATCC 30-2020
References: 39791: Lebron JA, et al. Tolerization of ***** mice to immunodominant proteins before monoclonal antibody production. J. Immunol. Methods 222: 59-63, 1999. PubMed: 10022372
39792: Lebron JA, et al. 3A5細(xì)胞Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Cell 93: 111-123, 1998. PubMed: 9546397
