SJD.1細胞
ATCC Number： CRL-2296?
細胞形態： 成纖維樣
數量： 大量
生長狀態： 貼壁生長
是否是腫瘤細胞： 0
物種來源： 斑馬魚
器官來源： 其他
品系： SJD
年限： *****
運輸方式： 凍存運輸
Designations: SJD.1
SJD.1細胞Depositors: BH Paw, LI Zon
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Danio rerio
Morphology: fibroblast
Source: Organ: caudal fin
Strain: SJD
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: *****
Comments: SJD.1細胞SJD.1 is a fibroblast cell line derived from amputated caudal fins of an ***** zebrafish, strain SJD.
This strain of zebrafish is used for genetic mapping.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Temperature: 28.0°C
SJD.1細胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with trypsin (0.25%)-EDTA (0.03%)-polyvinylpyrrolidone (0.5%) solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: SJD.1細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 43315: Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354
