3A(tPA-30-1)細胞
數量： 大量
細胞類型： SV40轉化細胞
是否是腫瘤細胞： 0
物種來源： 人
器官來源： 胎盤
生長狀態： 貼壁生長
運輸方式： 凍存運輸
ATCC Number： CRL-1583?
細胞形態： 上皮樣
Designations: 3A(tPA-30-1)
3A(tPA-30-1)細胞Depositors: J Chou
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: placenta
Cell Type: SV40 transformed
Cellular Products: at 40C the cells synthesize human chorionic gonadotropin (hCG) and alkaline phosphatase
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DNA Profile (STR): 3A(tPA-30-1)細胞Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis: This is a hypodiploid human cell line with the modal chromosome number of 42 occurring in 34% of cells. However, cells with 44 chromosomes also occurred at a high frequency (26%). Most cells contained 41-45 chromosomes. Cells having a higher ploidy occurred at 20.7%, which is very high. Most cells averaged five or six marker chromosomes, none of which appeared twice in the cells examined. No chromosomes were paired consistently.
Comments: The cells are transformed by SV40 ts30.
The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C).
The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
Propagation: 3A(tPA-30-1)細胞ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Temperature: 33.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 33?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 33?C to 34?C.
Normal placental functions are expressed when the cells are incubated at the restrictive temperature (40?C).
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: 3A(tPA-30-1)細胞Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
References: 1102: Chou JY. Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions. Proc. Natl. Acad. Sci. USA 75: 1409-1413, 1978. PubMed: 206898
