EN9F10細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
數量： 大量
器官來源： 脾
細胞形態： **樣
**類型： mouse IgG2b
ATCC Number： CRL-2403?
品系： BALB/c
運輸方式： 凍存運輸
Designations: EN9F10
EN9F10細胞Depositors: RA Shelby
Isotype: mouse IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: B lymphoblast;
Cellular Products: immunoglobulin; monoclonal antibody; against ergonovine (an ergot alkaloid)
Permits/Forms: EN9F10細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate.
The antibody detects ergopeptine and lysergic acid derivatives.
The antibody can be used to determine the toxic potential of agricultural commodities and forensic samples involving most ergot alkaloids.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 10(5) viable cells/ml.
Interval: EN9F10細胞Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 38309: Shelby RA, Kelley VC. Detection of ergot alkaloids in tall fescue by competitive immunoassay with a monoclonal antibody. Food Agric. Immunol. 3: 169-177, 1991.
38310: Shelby RA, Kelley VC. EN9F10細胞Detection of ergot alkaloids from Clavicops species in agricultural products by competitive ELISA using a monoclonal antibody. J. Agric. Food Chem. 40: 1090-1092, 1992.
