EOC 20細胞
年限： 10 days juvenile
運輸方式： 凍存運輸
細胞類型： 其他細胞類型
生長狀態： 貼壁生長
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： CRL-2469?
數量： 大量
器官來源： 大腦
品系： C3H/HeJ
細胞形態： 單核細胞/巨噬細胞
Designations: EOC 20
EOC 20細胞Depositors: WS Walker
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: macrophage
Source: Organ: brain
Strain: C3H/HeJ
Cell Type: microglia;
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Receptors: colony stimulating factor 1 (CSF-1R, CD115)
Antigen Expression: EOC 20細胞CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD45 +, Ly-6C +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR +, F4/80 +/-, CD86 (B7.2) - [39974]
Age: 10 days juvenile
Gender: female
Comments: This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse [PubMed: 8550814]. Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. The cell line is dependent on growth factor colony stimulating factor 1 (CSF-1). Conditioned medium is made from LADMAC cells (ATCC CRL-2420?) as a source of CSF-1.The cells exhibit phagocytic activity. These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens and expression was upregulated by recombinant murine interferon-gamma. The cells may be used to characterize the role of brain macrophages.C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove 75% of the media. Scrape off the attached cells and transfer into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: EOC 20細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
purified RNA:ATCC CRL-2469R
References: 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
39968: Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530
39974: Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814
39975: Askew D, Walker WS. EOC 20細胞Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775
