3B-11細胞
年限： *****
器官來源： 腋窩**結
生長狀態： 貼壁生長
品系： C3H/HeJ
組織來源： vascular epithelium
運輸方式： 凍存運輸
細胞類型： SV40轉化細胞
ATCC Number： CRL-2160?
是否是腫瘤細胞： 0
3B-11細胞物種來源： 小鼠
細胞形態： 上皮樣
數量： 大量
Designations: 3B-11
Depositors: KA O'Connell
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: axillary lymph node
Strain: C3H/HeJ
Tissue: 3B-11細胞vascular epithelium
Cell Type: endothelialSV40 transformed
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1992
Tumorigenic: Yes
Antigen Expression: H-2 K; VCAM
Age: *****
Gender: male
Comments: The 3B-11 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
3B-11 cells were cloned in 1992 by limiting dilution.
They are resistant to lysis by activated macrophages as measured in the chromium release assay.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).
The cells stain positively for SV40 T antigen.
Propagation: 3B-11細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: 3B-11細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170
23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612
23187: O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299
