ER4.7G.11 [7G11]細(xì)胞
ATCC Number： HB-11642?
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
**類(lèi)型： IgG2a kappa
數(shù)量： 大量
ER4.7G.11 [7G11]細(xì)胞生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，少量貼壁
Designations: ER4.7G.11 [7G11]
Depositors: Repligen Corporation
Isotype: IgG2a kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, with some loosely adherent cells
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source:ER4.7G.11 [7G11]細(xì)胞 Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with recombinant human CTLA4. Spleen cells were fused with?Monoclonal antibody ER4.7G11 stimulates CD8 positive T cells to proliferate [U.S. Pat. 5,858,358].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
ER4.7G.11 [7G11]細(xì)胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Shake off attached cells. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 70728: June CH, et al. ER4.7G.11 [7G11]細(xì)胞Methods for selectively stimulating proliferation of T cells. US Patent 5,858,358 dated Jan 12 1999
70730: Gray GS. CD9 antigen peptides and antibodies thereto. US Patent 5,883,223 dated Mar 16 1999
70733: June CH, et al. Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells. US Patent 6,352,694 dated Mar 5 2002
70734: June CH, et al. Methods for selectively stimulating proliferation of T cells. US Patent 6,534,055 dated Mar 18 2003
