3C10細胞
運輸方式： 凍存運輸
生長狀態： 懸浮生長
數量： 大量
細胞形態： **樣
**類型： IgG2b
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： TIB-228?
Designations: 3C10
Depositors: RM Steinman
3C10細胞Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human monocyte; against CD14; cytotoxic
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Tumorigenic: Yes
Comments: Animals were immunized with human peripheral blood monocytes.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antibody reacts with human monocytes but not with platelets, lymphocytes, dendritic cells, epithelial cells or fibroblasts.
The antibody appears to react with all tissue macrophages, and to differentiate between macrophages and dendritic cells.
The antibody the antibody immunoprecipitates gp55.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 3C10細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1194: Van Voorhis WC, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J. Exp. Med. 158: 126-145, 1983. PubMed: 6190974
1195: Van Voorhis WC, et al. Relative efficacy of human monocytes and dendritic cells as accessory cells for T cell replication. J. Exp. Med. 158: 174-191, 1983. PubMed: 6190976
1318: Zvaifler NJ, et al. 3C10細胞Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis. J. Clin. Invest. 76: 789-800, 1985. PubMed: 3875632
