QCRL-1細胞
運輸方式： 凍存運輸
器官來源： 脾
生長狀態： 懸浮生長
ATCC Number： HB-11765?
數量： 大量
細胞形態： **樣
**類型： mouse IgG1 kappa
是否是腫瘤細胞： 0
物種來源： 小鼠
Designations: QCRL-1
Depositors: Queen's University at Kingston
Isotype: mouse IgG1 kappa
QCRL-1細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a multidrug resistance protein (MRP)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the multidrug resistant, small cell lung carcinoma, cell line H69AR (ATCC CRL-11351).
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody reacts with MRP (multidrug resistance protein). QCRL-1細胞MRP is a 190,000 integral membrane phosphoglycoprotein that is overexpressed in some drug-selected resistant cell lines and has been shown to cause multidrug resistance in transfected cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Attached cells can be scraped.
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can beestablished by centrifugation with subsequent resuspension in fresh medium at 2 to 5 X 10(5) viable cells/ml.
Interval: Maintain cultures at cell concentrations between 2 X 10(5) and 2 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: QCRL-1細胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 49655: Deeley RG, Cole SP. Antibodies to a multidrug resistance protein. US Patent 6,063,621 dated May 16 2000
49661: Hipfner DR, et al. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 54: 5788-5792, 1994. QCRL-1細胞PubMed: 7954400
