RPMI 1846細胞
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
器官來源： 皮膚
是否是腫瘤細胞： 0
物種來源： 其他
細胞形態(tài)： 上皮樣
數(shù)量： 大量
ATCC Number： CCL-49?
相關**： 其他**
Designations: RPMI 1846
Depositors: GE Moore
RPMI 1846細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mesocricetus auratus
Morphology: epithelial
Source: Organ: skin
Disease: melanoma, melanotic
Cellular Products: melanin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: Vesicular stomatitis virus
Human poliovirus 1
Tumorigenic: RPMI 1846細胞Yes
Cytogenetic Analysis: modal number = 67; range = 61 to 69
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: RPMI 1846細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 874: Moore GE, et al. Continuous culture of a melanotic cell line from the golden hamster. Science 137: 986-987, 1962. PubMed: 14475649
875: Mount D, et al. Culture of malignant tumors of the Syrian hamster. J. Natl. Cancer Inst. 31: 1217-1237, 1963. PubMed: 14071829
26206: Hsu TC, Kellogg DS Jr.. Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens. J. Natl. Cancer Inst. 25: 221-235, 1960. PubMed: 14403619
26207: Fortner JG, et al. Transplantable tumors of the Syrian (golden) hamster. I. Tumors of the alimentary tract, endocrine glands and melanomas. Cancer Res. 21: 161-198, 1961. RPMI 1846細胞PubMed: 13700922
