30-C7細胞
生長狀態： 懸浮生長
運輸方式： 凍存運輸
ATCC Number： TIB-106?
是否是腫瘤細胞： 0
物種來源： 大鼠
數量： 大量
細胞形態： **樣
**類型： IgG2a
Designations: 30-C7
Depositors: JA Ledbetter
Isotype: IgG2a
30-C7細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; 30-C7細胞monoclonal antibody; against Lgp100a (a 100000 dalton glycoprotein that is expressed on mouse T and B cells)
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Comments: Animals were immunized with mouse splenic or thymic lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 30-C7細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 58000: Ledbetter JA, Herzenberg LA. 30-C7細胞Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol. Rev. 47: 63-90, 1979. PubMed: 398327
