ECC-1 [EnCA1]細胞
器官來源： 其他
數量： 大量
生長狀態： 貼壁生長
細胞形態： 上皮樣
運輸方式： 凍存運輸
ATCC Number： CRL-2923?
是否是腫瘤細胞： 0
物種來源： 人
組織來源： luminal epithelium
Designations: ECC-1 [EnCA1]
Depositors: BA Lessey
ECC-1 [EnCA1]細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: endometrium adenocarcinoma
Tissue: luminal epithelium
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Receptors: androgen receptor, expressed
estrogen receptor 1 (ER alpha), expressed
estrogen receptor 2 (ER beta), expressed
progesterone receptor A, expressed
progesterone receptor B, expressed
interferon-gamma (IFN-gamma), expressed
ECC-1 [EnCA1]細胞Antigen Expression: cytokeratin-18; Homo sapiens, expressed
cytokeratin-13; Homo sapiens, expressed
epithelial membrane antigen; Homo sapiens, expressed
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17
Comments: EEC-1 was developed by Satyaswaroop by passing a well differentiated adenocarcinoma of human endometrial lining into nude mice. [PubMed; 6849115].The cells exhibit Epithelial Growth Factor (EGF-)-stimulated expression of known luminal proteins. This cell line can be used to study endocrine and paracrine regulation of endometrial epithelium in vitro. [PubMed: 16707768].
Propagation: ECC-1 [EnCA1]細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
ECC-1 [EnCA1]細胞Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 6 X 10(3) to 1 X 10(4) viable cells/sq. cm is recommended.
Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 2 X 10(5) and 5 X 10(5) cells/sq. cm.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium,95%;DMSO,5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 26 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
phosphate-buffered saline:ATCC 30-2200
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
References: 92849: Mo B, et al. ECC-1 [EnCA1]細胞ECC-1 cells: a well-differentiated steroid-responsive endometrial cell line with characteristics of luminal epithelium. Biol. Reprod. 75: 387-394, 2006. PubMed: 16707768
92850: Satyaswaroop PG, et al. Human endometrial adenocarcinoma transplanted into nude mice: growth regulation by estradiol. Science 219: 58-60, 1983. PubMed: 6849115
92851: Tabibzadeh S, et al. Human endometrial epithelial cell lines for studying steroid and cytokine actions. In Vitro Cell. Dev. Biol. 26: 1173-1179, 1990. PubMed: 1706699
92852: Ricci MS, et al. ECC-1 human endometrial cells as a model system to study dioxin disruption of steroid hormone function. In Vitro Cell. Dev. Biol. Anim. 35: 183-189, 1999. PubMed: 10478797
92853: Castro-Rivera E, et al. Estrogen and aryl hydrocarbon responsiveness of ECC-1 endometrial cancer cells. Mol. Cell. Endocrinol. 150: 11-21, 1999. PubMed: 10411295
