LB3.1細胞

數(shù)量： 大量

細胞形態(tài)： **樣

**類型： IgG2a

ATCC Number： HB-298?

生長狀態(tài)： 懸浮生長

是否是腫瘤細胞： 0

物種來源： 小鼠

運輸方式： 凍存運輸

Designations: LB3.1

LB3.1細胞Depositors: JL Strominger

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; LB3.1細胞monoclonal antibody; against HLA-DR alpha chain

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Comments: Animals were immunized with purified HLA-DR protein.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with a conformational epitope on the alpha chain of HLA-DR.

Propagation: LB3.1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23208: Gorga JC, et al. Immunochemically purified DR antigens in liposomes stimulate xenogeneic cytolytic T cells in secondary in vitro cultures. Cell. Immunol. 103: 160-173, 1986. LB3.1細胞PubMed: 3492282