TS2/18.1.1細胞

細胞形態： **樣

**類型： mouse IgG1

生長狀態： 懸浮生長

數量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

運輸方式： 凍存運輸

ATCC Number： HB-195?

器官來源： 脾

Designations: TS2/18.1.1

Depositors: TA Springer

TS2/18.1.1細胞Isotype: mouse IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Cell Type: hybridoma: B lymphocyte;

Cellular Products:TS2/18.1.1細胞 immunoglobulin; monoclonal antibody; against lymphocyte function antigen 2 (LFA-2); against CD2

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [1616]

Comments: Animals were immunized with human cytolytic T lymphocytes.

Spleen cells were fused with NS-1 myeloma cells.

The antibody will inhibit HLA DR mediated T cell cytotoxicity.

The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. TS2/18.1.1細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.

Interval: Maintain cell density between 5 X 10(4) and 2 X 10(5) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1616: Sanchez-Madrid F, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489-7493, 1982. TS2/18.1.1細胞PubMed: 6984191