E.G7-OVA細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： CRL-2113?
相關(guān)**： **瘤
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
品系： C57BL/6N
細(xì)胞類型： T**細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
E.G7-OVA細(xì)胞Designations: E.G7-OVA [derivative of EL4 (see ATCC TIB-39)]
Depositors: MJ Bevan
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6N
Cell Type: T lymphocyte;
Cellular Products: chicken ovalbumin
Permits/Forms: E.G7-OVA細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2 b
Comments: E.G7-OVA was derived in 1988 from the C57BL/6 (H-2 b) mouse lymphoma cell line EL4 (ATCC TIB-39).
The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene.
E.G7-OVA cells contain a single copy of the inserted plasmid and synthesize and secrete OVA constitutively.
C57BL/6 mice immunized with E.G7-OVA cells give rise to H-2 Kb restricted cytotoxic lymphocytes specific for the OVA 258-276 peptide.
This cell line is a model system for studying major histocompatibility complex (MHC) class I restricted responses of cytotoxic T lymphocytes in mice.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
E.G7-OVA細(xì)胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC TIB-39
References: 22630: Moore MW, et al. E.G7-OVA細(xì)胞Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777-785, 1988. PubMed: 3261634
