TA-1細胞
生長狀態(tài)： 懸浮生長
ATCC Number： HB-10206?
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
數(shù)量： 大量
品系： BALB/c
細胞形態(tài)： **樣
**類型： mouse IgG1 kappa
Designations: TA-1
Depositors: WP Carney
TA-1細胞Isotype: mouse IgG1 kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Strain: BALB/c
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: TA-1細胞Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. Spleen cells were fused with SP2/O myeloma cells (ATCC CRL 1581). The antibody recognizes substantially purified p100, human neu related protein, the full-length HER-2/neu molecule in tumor tissue (p185) or the extracellular domain (ECD) (p105) in serum, plasma, cell cultures and fluids. The TA-1 (HB-10206) and NB-3 (HB-10205) antibodies recognize two distinct epitopes on the human neu gene product [U.S. Pat. 5,401,638].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Maintain cultures at a cell concentration between 5 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 38764: TA-1細胞Bacus SS, et al. Differentiation of cultured human breast cancer cells (AU-565 and MCF-7) associated with loss of cell surface HER-2/neu antigen. Mol. Carcinog. 3: 350-362, 1990. PubMed: 1980588
72026: Carney WP, McKenzie SJ. Detection and quantification of neu related proteins in the biological fluids of humans. US Patent 5,401,638 dated Mar 28 1995
72027: Carney WP, et al. Detection of neu p185 in cell lysates. US Patent 5,604,107 dated Feb 18 1997
89003: Rodrquez GC, et al. The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines. Am. J. Obstet. Gynecol. 168: 228-232, 1993. PubMed: 8420332
89139: McKenzie SJ, et al. Generation and characterization of monoclonal antibodies specific for the human neu oncogene product, p185. Oncogene 4: 543-548, 1989. PubMed: 2566965
89140: Singleton TP, et al. TA-1細胞Detection of c-erbB-2 activation in paraffin-embedded tissue by immunohistochemistry. Hum. Pathol. 23: 1141-1150, 1992. PubMed: 1356909
