RIN-5F細胞
運輸方式： 凍存運輸
ATCC Number： CRL-2058?
品系： NEDH
組織來源： islet cell tumor
生長狀態： 貼壁生長
細胞形態： 上皮樣
器官來源： 胰腺
數量： 大量
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
RIN-5F細胞物種來源： 大鼠
Designations: RIN-5F
Depositors: AF Gazdar, H Oie
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: pancreas
Strain: NEDH
Tissue: islet cell tumor
Cell Type: beta cell;
Cellular Products: insulin; L-dopa-decarboxylase
Permits/Forms: RIN-5F細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments: The RIN-5F cell line is a clone derived from the RIN-m rat islet line (ATCC CRL-2057 ). The cells produce and secrete insulin, and produce L-dopa-decarboxylase (a marker for cells having amine precursor uptake and decarboxylation, or APUD, activity). Unlike the parental line they do not produce somatostatin. The parental line was also cloned to establish RIN-14B (ATCC CRL-2059) that produces only somatostatin. These offer models for the study of the biology of pancreatic islet cells, specifically the mechanisms controlling the synthesis, storage and secretion of insulin and somatostatin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Monolayer never becomes confluentVolumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).4. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.5. RIN-5F細胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 6. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.7. Discard supernatant and resuspend cells in fresh growth medium8. Add appropriate aliquots of the cell suspension to new culture vessels. 9. Incubate cultures at 37?C. Cells will take about 3 days to attach fully
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2057
References: 22483: RIN-5F細胞Bhathena SJ, et al. Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones. Proc. Soc. Exp. Biol. Med. 175: 35-38, 1984. PubMed: 6141566
22592: Chick WL, et al. A transplantable insulinoma in the rat. Proc. Natl. Acad. Sci. USA 74: 628-632, 1977. PubMed: 191819
22640: Bhathena SJ, et al. Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor. Diabetes 31: 521-531, 1982. PubMed: 6295859
23266: Gazdar AF, et al. Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc. Natl. Acad. Sci. USA 77: 3519-3523, 1980. PubMed: 6106192
23511: Oie HK, et al. Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor. Endocrinology 112: 1070-1075, 1983. PubMed: 6129963
