WR19M.1細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
數量： 大量
生長狀態： 貼壁生長
運輸方式： 凍存運輸
ATCC Number： TIB-70?
相關**： 其他**
品系： BALB/c
Designations: WR19M.1
WR19M.1細胞Depositors: WC Raschke
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Disease: Abelson murine leukemia virus-induced tumor
Strain: BALB/c
Cellular Products: lysozyme; Abelson murine leukemia virus (infectious)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. WR19M.1細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: complement (C3)
Antigen Expression: H-2d
Gender: female
Comments: This line was derived from the same tumor as WR19L (ATCC TIB-52).
The cells will pinocytose neutral red and phagocytose zymosan and latex beads.
They will also lyse antibody coated sheep erythrocytes.
They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2-).
WR19M.1細胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 1 to 2 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum: ATCC 30-2040
References: 1207: Raschke WC, et al. WR19M.1細胞Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
