DS1細胞
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
ATCC Number： CRL-2484?
生長狀態： 懸浮生長
細胞形態： **樣
**類型： mouse IgG1 kappa
Designations: DS1
Depositors: DG Sieckmann
DS1細胞Isotype: mouse IgG1 kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Cellular Products: immunoglobulin; monoclonal antibody; against mouse IgMa allotype
Permits/Forms: DS1細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.
Isolation: Isolation date: 1986
Comments: Animals were immunized against TC31, a NMRI strain mouse IgM, kappa monoclonal antibody of the IgMa allotype.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes the Igh-6.1 allotype on mouse IgM.
It reacts with the IgMa but not the IgMb determinant localized to the CH1 domain of the mu heavy chain. [40006]
DS1 antibody reacts with cells bearing the a,c,f,g,h and j Igh-C haplotypes.
DS1細胞This antibody can be used as a typing reagent for mouse IgM of the a,c,f,g,h and j haplotypes. It is useful in typing immunoglobulin transgenic mice in which the IgM locus is involved.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 4 to 5 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: DS1細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 40006: Sieckmann DG, et al. A mouse monoclonal antibody specific for an allotypic determinant of the Igha allele of murine IgM: genetic and functional analysis of Igh-6a epitopes using anti-IgM monoclonal antibodies. Hybridoma 10: 121-135, 1991. PubMed: 1709612
47302: Sieckmann D. A monoclonal mouse antibody that recognizes a mouse IgM allotypic determinant (IgH-6.1). Fed. Proc. 46: 488, 1987.
