1116NS-3d細胞
細胞形態： **樣
**類型： IgM
生長狀態： 懸浮生長
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
ATCC Number： CRL-8019?
Designations: 1116NS-3d
Depositors: Wistar Institute
Isotype: IgM
1116NS-3d細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: 1116NS-3d細胞Animals were immunized with a human colonic adenocarcinoma (SW1116, ATCC CCL-233).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing: 1116NS-3d細胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3509: Koprowski H, et al. 1116NS-3d細胞Monoclonal hybridoma antibody specific for high molecular weight carcinoembryonic antigen. US Patent 4,349,528 dated Sep 14 1982
