Cf2Th細胞
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
ATCC Number： CRL-1430?
相關(guān)**： 正常
是否是腫瘤細胞： 0
物種來源： 狗
年限： newborn
數(shù)量： 大量
器官來源： 胸腺
Cf2Th細胞Designations: Cf2Th
Depositors: WA Nelson-Rees
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Canis familiaris
Morphology:
Source: Organ: thymus
Disease: normal
Permits/Forms: Cf2Th細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Age: newborn
Gender: female
Comments: The line has been used to propagate retroviruses from cat embryo and baboon lung and kidney.
The cells are also susceptible to xenotropic retroviruses from AKR and BALB/c mice.
Propagation: Cf2Th細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with non-essential amino acids, 80%; fetal bovine serum, 20%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22649: Nelson-Rees WA, et al. Source, alterations, characteristics and use of a new dog cell line (Cf2Th). In Vitro 12: 665-669, 1976. PubMed: 190163
32500: Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326
32728: Russell DW, Miller AD. Foamy virus vectors. J. Cf2Th細胞Virol. 70: 217-222, 1996. PubMed: 8523528
