BSC40 [BSC-40]細胞
器官來源： 腎臟
細胞類型： 上皮細胞
是否是腫瘤細胞： 0
物種來源： 綠長尾猴
數量： 大量
生長狀態： 貼壁生長
細胞形態： 上皮樣
ATCC Number： CRL-2761?
運輸方式： 凍存運輸
Designations: BSC40 [BSC-40]
Depositors: RC Condit
BSC40 [BSC-40]細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cercopithecus aethiops
Morphology: epithelial
Source: Organ: kidney
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: BSC40 [BSC-40]細胞This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit
Applications: virus host (BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31C to 40C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies.)
Virus Susceptibility: Vaccinia virus
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Max Temperature: 40.0°C
Min Temperature: 31.0°C
Subculturing: BSC40 [BSC-40]細胞Protocol: Subculture at confuency. Do not allow cells to overgrow.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 61336: BSC40 [BSC-40]細胞Condit RC, Motyczka A. Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus. Virology 113: 224-241, 1981. PubMed: 7269240
61337: Brockman WW, Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc. Natl. Acad. Sci. USA 71: 942-946, 1974. PubMed: 4362640
61338: Ensinger MJ. Isolation and genetic characterization of temperature-sensitive mutants of vaccinia virus WR. J. Virol. 43: 778-790, 1982. PubMed: 7143565
