R2C細(xì)胞
年限： 2 months
細(xì)胞形態(tài)： 上皮樣
器官來源： 睪丸
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
運(yùn)輸方式： 凍存運(yùn)輸
品系： WFu
ATCC Number： CCL-97?
相關(guān)**： 其他**
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
Designations: R2C
R2C細(xì)胞Depositors: G Sato
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: testis
Strain: WFu
Disease: Leydig cell tumor
Cellular Products: steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. R2C細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Virus Resistance: poliovirus 2
Reverse Transcript: positive
Age: 2 months
Gender: male
Comments: Stimulation by other hormones or cAMP is not required for hormone secretion.
Propagation: ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 2.5%
horse serum to a final concentration of 15%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Add fresh medium at least twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. R2C細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. R2C細(xì)胞PubMed: 4868022
