IM-9細(xì)胞
ATCC Number： CCL-159?
器官來(lái)源： 外周血
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞類(lèi)型： B**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
IM-9細(xì)胞**類(lèi)型： IgG kappa , IgG; kappa light chain [26021]
Designations: IM-9
Depositors: DN Buell
Isotype: IgG kappa , IgG; kappa light chain [26021]
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: IM-9細(xì)胞lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Cellular Products: immunoglobulin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1967
Receptors: growth hormone
insulin
calcitonin
growth hormone; insulin; calcitonin
Antigen Expression: CD11a +; CD19 +; CD20 +; CD38 -; CD49e +
DNA Profile (STR): IM-9細(xì)胞Amelogenin: X
CSF1PO: 10,11
D13S317: 9,11
D16S539: 9,13
D5S818: 13
D7S820: 11,12
THO1: 6,9.3
TPOX: 11
vWA: 14,17
Cytogenetic Analysis: normal human female; diploid; stable
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 0
Gender: female
Ethnicity: Caucasian
Comments: Although derived from the blood of a patient with multiple myeloma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]
The cells are EBNA positive.
Propagation: IM-9細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1-2 X 10 exp5 viable cells/ml.Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Do not allow the cell density to exceed 2 X 10 exp6 cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1031: Fahey JL, et al. Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann. N.Y. Acad. Sci. 190: 221-234, 1971. PubMed: 5290016
1035: Lesniak MA, et al. Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J. Biol. Chem. 249: 1661-1667, 1974. PubMed: 4361817
1225: Van Obberghen E, et al. Cell surface receptors for insulin and human growth hormone. Effect of microtubule and microfilament modifiers. J. Biol. Chem. 251: 6844-6851, 1976. PubMed: 185222
1226: Gavin JR, et al. Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc. Natl. Acad. Sci. USA 71: 84-88, 1974. PubMed: 4359334
26021: van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
