Pro-5細胞
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 倉鼠
ATCC Number： CRL-1781?
運輸方式： 凍存運輸
生長狀態(tài)： 單層（少量懸浮）
器官來源： 卵巢
細胞形態(tài)： 上皮樣
Designations: Pro-5
Depositors: P Stanley
Pro-5細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: monolayer or suspension
Organism: Cricetulus griseus
Morphology: epithelial
Source: Organ: ovary
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Pro-5細胞The parental Pro-5 line was deposited by Dr. P. Stanley. The cells are not available commercially and it is requested that: 1) In all papers using the cell line a direct reference will be made to Stanley et al. Cell 6:121-128, 1975. 2) Any proposed commercial use of the cells must be negotiated with Dr. P. Stanley, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA. 3) No distribution of line derived from the Pro-5 line (including those derived by hybridization and/or transfection) be made to third parties without Dr. Stanley's permission
Gender: female
Comments: This line is a derivative of the original CHO cell line and is the parental line for several glycosylation mutants (see ATCC CRL-1735, 1736 and 1737).
The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at high frequency (approximately 1 in 250 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.
Propagation: Pro-5細胞ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature:Pro-5細胞 liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
References: 1201: Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798
