FRTL細胞
ATCC Number： CRL-1468?
相關**： 正常
器官來源： 甲狀腺
生長狀態： 貼壁生長
品系： Fischer
年限： 6 weeks
是否是腫瘤細胞： 0
物種來源： 大鼠
運輸方式： 凍存運輸
數量： 大量
FRTL細胞細胞形態： 上皮樣
Designations: FRTL
Depositors: HG Coon
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, patchy
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Strain: Fischer
Organ: thyroid
Disease: normal
Cellular Products: thyroglobulin
Permits/Forms: FRTL細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Cytogenetic Analysis: diploid
Age: 6 weeks
Comments: The cells accumulate iodide intracellularly.
Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with:10 mU/ml TSH, 0.01 mg/ml insulin10 nM hydrocortisone 0.005 mg/ml transferrin10 ng/ml somatostatin10 ng/ml glycyl-L-histidyl-L-lysine acetate0.5 % bovine calf serum(All are obtainable from Sigma, Collaborative Research and/or Calbiochem.)
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: FRTL細胞Protocol: There is an absolute requirement for 0.1 to 0.5% calf serum in addition to insulin, hydrocortisone, thyrotropin, transferrin, somatostatin and glycyl-1-histidyl-lysine acetate.
The cells tend to grow one above another, forming three dimensional structures rather than expanding into a monolayer.
Subculture before ?domes? form, flasks never become confluent.
Remove and discard culture medium.
Add 2.0 to 3.0 ml of CTC solution ( Collagenase, 20 U/ml; Trypsin, 0.075%; dialyzed heat-inactivated Chicken serum, 2%) to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-collagenase solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.
Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Place culture vessels in incubators at 37?C.
Subcultivation Ratio: FRTL細胞A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2030
References: 1066: Ambesi-Impiombato FS, et al. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc. Natl. Acad. Sci. USA 77: 3455-3459, 1980. PubMed: 6106191
23211: Bidey SP, et al. Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro bioassay system for thyrotrophin. J. Endocrinol. 101: 269-276, 1984. PubMed: 6327870
