MAVER-1細胞
細胞類型： **母細胞
是否是腫瘤細胞： 0
物種來源： 人
ATCC Number： CRL-3008?
相關**： 其他**
生長狀態： 懸浮生長
數量： 大量
器官來源： 外周血
細胞形態： **樣
年限： 77 years
運輸方式： 凍存運輸
MAVER-1細胞Designations: MAVER-1
Depositors: A Zamo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Characterization Data
Source: Organ: peripheral blood
Cell Type: lymphoblast
Disease: MAVER-1細胞Mantle Cell Lymphoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 2003
Antigen Expression: CD3-, CD5+, CD10-, CD19+, CD20+, CD23-, FMC7+ (verified at ATCC )
DNA Profile (STR): Amelogenin: X Y
CSF1PO: 10, 11
D13S317: 8, 13
D16S539: 9, 12
D5S818: 12
D7S820: 8
THO1: 8, 9
TPOX: 8, 12
MAVER-1細胞vWA: 14, 18
Cytogenetic Analysis: This is a hypodiploid cell line of male origin with a chromosome number range of 38-40. The karyotype is highly rearranged and although there has been a loss of chromosome number, most of the cells still contain several of the derivative chromosomes originally described in the literature. Specifically, the t(11;14) derivative common to mantle cell lymphoma is present in a complex rearrangement with chromosome number 6, der(6)t(6;14;11)(q13;q11.2?;q23?) and was confirmed with whole chromosome paint probes for chromosomes 11 and 14. Other derivatives include add(1)(p36.1), add(4)(q31) and del(11)(q13).
Age: 77 years
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(5) to 5 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 2 X 10(6) cells/ml.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:5 is recommended.
Medium renewal: every 2 to 3 days depending on cell density
Preservation:MAVER-1細胞 Freeze medium: complete growth medium supplemented with an additional 10% fetal bovine serum and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: approximately 24 hours
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
Cell culture tested DMSO: ATCC 4-X
References: 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16173255: Zamo A, et al. Establishment of the MAVER-1 cell line, a model for leukemic and aggressive mantle cell lymphoma. Haematologica: 91(1):40-47, 2006. PubMed: 16434369
