EGC/PK060399egfr細(xì)胞
器官來源： 其他
細(xì)胞類型： 其他細(xì)胞類型
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 褐鼠
品系： Sprague-Dawley
組織來源： myenteric plexus
數(shù)量： 大量
EGC/PK060399egfr細(xì)胞細(xì)胞形態(tài)： 其他
年限： ***** *****
ATCC Number： CRL-2690?
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
Designations: EGC/PK060399egfr
Depositors: A Ruhl
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: EGC/PK060399egfr細(xì)胞Rattus rattus deposited as rat
Morphology: glial
Source: Organ: jejunum
Strain: Sprague-Dawley
Tissue: myenteric plexus
Cell Type: enteroglial;
Cellular Products: glial fibrillary acidic protein (GFAP); S-100 protein and vimentin [53238]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: March 6, 1999
EGC/PK060399egfr細(xì)胞Age: ***** *****
Gender: male
Comments: The cells display robust glial fibrillary acidic protein (GFAP), S-100 and vimentin immunoreactivities, but no Thy-1.1, desmin, smooth muscle alpha-actin or C3 complement receptor immunoreactivity. [53238]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: EGC/PK060399egfr細(xì)胞Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 53238: Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of ***** rat. Neurogastroenterol. Motil. 13: 95-106, 2001. EGC/PK060399egfr細(xì)胞PubMed: 11169131
