FAT 7細胞
運輸方式： 凍存運輸
年限： *****
ATCC Number： CRL-2109?
數(shù)量： 大量
相關**： 鱗狀細胞癌
組織來源： nasal
生長狀態(tài)： 貼壁生長
細胞形態(tài)： 上皮樣
是否是腫瘤細胞： 0
物種來源： 大鼠
Designations: FAT 7
Depositors: E Bermudez
FAT 7細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Tissue: nasal
Disease: squamous cell carcinoma
Cellular Products: transforming growth factor alpha (TGF alpha)
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Receptors: epidermal growth factor (EGF)
Tumorigenic: FAT 7細胞Yes
Age: *****
Gender: male
Comments: The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an ***** male rat.
The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion).
Propagation: ATCC complete growth medium: Ham's F12K medium with 0.01 mg/ml insulin, 250 ng/ml hydrocortisone and 0.0025 mg/ml transferrin, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
FAT 7細胞Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add 1 to 2 ml of additional trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 24 hrs
References: 22639: Bermudez E, et al. Characterization of cell lines derived from formaldehyde-induced nasal tumors in rats. Mol. Carcinog. 9: 193-199, 1994. FAT 7細胞PubMed: 8148052
