4F2C13細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

數(shù)量： 大量

ATCC Number： HB-22?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

4F2C13細(xì)胞生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: 4F2C13

Depositors: A Fauci, BF Haynes

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: 4F2C13細(xì)胞lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human monocyte

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line).

Spleen cells were fused with P3X63Ag8 myeloma cells.

This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.

4F2C13細(xì)胞The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells.

The antibody fixes complement and binds to Protein A.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; 4F2C13細(xì)胞carbon dioxide (CO2), 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium.

Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

References: 916: Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. 4F2C13細(xì)胞PubMed: 6153676