B27M2細胞
數量： 大量
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgM
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
ATCC Number： HB-165?
Designations: B27M2
Depositors: F Grumet
Isotype: IgM
Biosafety Level: 1
B27M2細胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
B27M2細胞
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic
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Comments: Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for a subpopulation of HLA B27 positive cells and for all HLA Bw47 positive cells at an appropriate titer.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Modified RPMI 1640 medium (see fact sheet), 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1171: B27M2細胞Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637
