NCI-H676B細胞
年限： 63 years
運輸方式： 凍存運輸
生長狀態： 懸浮生長
細胞形態： 上皮樣
器官來源： 肺
ATCC Number： HTB-179?
相關**： 腺癌
數量： 大量
是否是腫瘤細胞： 0
物種來源： 人
Designations: NCI-H676B [H676B]
Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
NCI-H676B細胞Growth Properties: suspension, multicell aggregates (gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures)
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion
Cellular Products: mucin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 9
D13S317: 10
D16S539: 11,12
D5S818: 11
D7S820: 12
THO1: 9.3
TPOX: 8,11
vWA: 16,19
Cytogenetic Analysis: This is a hypodiploid cell line; modal number = 43; range = 39 to 47. Marker chromosomes consisted of over 70% of the chromosomes in each cell complement. Among the identifiable markers were t(1q8q), der(3)t(3;?)(q29;?), t(2q7q), der(9)t(9;14)(p13;q11) and der(11)t(?q23;?). NCI-H676B細胞All markers had a single copy per cells. The N5, N9, N10, N17, N18, N20, N21 and the X were the only structurally normal chromosomes found. Except for the paired N5, all others had single copies per cell.
Isoenzymes: AK-1, 2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 1-2
PGM3, 2
Age: 63 years
Gender: male
Ethnicity: Caucasian
Comments: The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb).
The line does not exhibit any gross structural DNA abnormalities.
The cells produce mucin, and occasionally signet ring cells are formed.
Propagation: NCI-H676B細胞ATCC complete growth medium: ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite (final conc.)
50 nM Hydrocortisone (final conc.)
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine (final conc.)
0.01 mM phosphorylethanolamine (final conc.)
100 pM triiodothyronine (final conc.)
0.5% (w/v) bovine serum albumin (final conc.)
0.5 mM sodium pyruvate (final conc.)
extra 2mM L-glutamine (for final conc. of 4.5mM)
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.
Subcultivation Ratio: NCI-H676B細胞A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 82.5%; fetal bovine serum, 10%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
References: 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
