4D1.5.7細胞
ATCC Number： HB-11495?
數量： 大量
生長狀態： 懸浮生長
運輸方式： 凍存運輸
細胞形態： **樣
是否是腫瘤細胞： 0
物種來源： 小鼠
**類型： IgG2a
Designations: 4D1.5.7
Depositors: Genentech, Inc.
Isotype: IgG2a
4D1.5.7細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against interleukin 8 receptor type B (IL-8R-B, CDw128)
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Comments: 4D1.5.7細胞Animals were immunized with the 293-27 cell line (human fetal kidney, transfected to express IL-8 receptor type B).
Spleen cells were fused with P3X63Ag8U.1 myeloma cells.
The antibody binds to IL-8R-B with Kd = 1.9 nM.
It does not bind to the type A receptor.
The epitope recognized by 4D1.5.7 appears to be expressed in the N terminal portion of the molecule.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 4D1.5.7細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22079: Chuntharapai A, et al. Antibodies to human IL-8 type B receptor. US Patent 5,440,021 dated Aug 8 1995
23489: Chuntharapai A, et al. Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes. J. Immunol. 153: 5682-5688, 1994. PubMed: 7527448
23490: 4D1.5.7細胞Chuntharapai A, Kim KJ. Regulation of the expression of IL-8 receptor A/B by IL-8: possible functions of each receptor. J. Immunol. 155: 2587-2594, 1995. PubMed: 7650389
