LLC-MK2 Derivative細胞
器官來源： 腎臟
年限： *****
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
ATCC Number： CCL-7.1?
相關**： 正常
運輸方式： 凍存運輸
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 獼猴
LLC-MK2 Derivative細胞Designations: LLC-MK2 Derivative
Depositors: RN Hull
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Macaca mulatta
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Cellular Products: plasminogen activator; keratin
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Virus Susceptibility: LLC-MK2 Derivative細胞Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Cytogenetic Analysis: modal number = 70; range = 41 to 136.
No marker chromosomes noted.No marker chromosomes noted.
Age: *****
Comments: LLC-MK2 Derivative細胞The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: LLC-MK2 Derivative細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
