Mino細(xì)胞
年限： 64
組織來源： Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
器官來源： 外周血
生長狀態(tài)： 懸浮生長
ATCC Number： CRL-3000?
相關(guān)**： 其他**
細(xì)胞形態(tài)： **樣
運輸方式： 凍存運輸
數(shù)量： 大量
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 人
Designations: Mino
Mino細(xì)胞Depositors: R.J.Ford
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Characterization Data
Source: Organ: peripheral blood
Tissue: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
Cell type: lymphoblast; cytogenetic abnormality
Disease: Mantle Cell Lymphoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: The immunophenotype by flow cytometry is compatible with MCL [PubMed: 12127561].
Antigen Expression: CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC )
DNA Profile (STR): Mino細(xì)胞Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 10,11
THO1: 9.3
TPOX: 8,11
vWA: 14,17
Cytogenetic Analysis: Modal chromosome number: 74. This is a hypertriploid male cell line, del(6)(q16), t(8;11)(q22;q23), add(11)(q25), add(13)(q34), add(14)(q32). Most cells contain the t(11;14) derivative chromosome, detected by FISH with whole chromosome paint probes.
Age: 64
Gender: male
Ethnicity: Caucasian
Comments: The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL [PubMed: 12127561].Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1) [PubMed: 12127561].
Propagation: Mino細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 1.5 X 10(6) cells/ml. Subcultivation Ratio: 1:5 to 1:10.
Preservation: 90% FBS; 10% (v/v) DMSO
Doubling Time: about 72 hours
References: 92889: Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561
92890: Marzec M, et al. Mantle cell lymphoma cells express predominantly cyclin D1a isoform and are highly sensitive to selective inhibition of CDK4 kinase activity. Blood 108: 1744-1750, 2006. PubMed: 16690963
92895: Salaverria I, et al. Mino細(xì)胞Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
