91MS441細胞
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgG1; kappa light chain
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-11301?
Designations: 91MS441
Depositors: University of Massachusetts
Isotype: IgG1; kappa light chain
91MS441細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
91MS441細胞Cellular Products: immunoglobulin; monoclonal antibody; against an antibody to a chlamydia genus antigen (anti-idiotypic antibody)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with 89MS30 (GLXA-Ab1) IgG.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Propagation: 91MS441細胞ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 40475: MacDonald B, et al. 91MS441細胞Oral vaccine comprising anti-idiotypic antibody to chlamydia glycolipid exoantigen and process. US Patent 5,656,271 dated Aug 12 1997
