27kDa熱休克蛋白(HSPB1)單克隆抗體

• 來源單抗制備
• 宿主小鼠
• 效價(克隆號)C4
• Ig亞型IgG1 Kappa
• 27kDa熱休克蛋白(HSPB1)單克隆抗體純化方式蛋白A + 蛋白G親和純化
• 標記物無標記物
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度500ug/ml
• 且適物種-
• 應用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
• 27kDa熱休克蛋白(HSPB1)單克隆抗體Monoclonal Antibody to Heat Shock Protein 27 (HSP27)
  Organism Species: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  11th Edition (Revised in May, 2016)
  [ PROPERTIES ]
  Source: Monoclonal antibody preparation
  Host: Mouse
  Antibody isotype: IgG1 Kappa
  Purification: Protein A/G Affinity Chromatography. Clone number: C4
  Traits: Liquid
  Concentration: 500μg/mL
  UOM: 200μg
  Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
  Immunogen: RPA693Hu01-Recombinant Heat Shock Protein 27 (HSP27)
  27kDa熱休克蛋白(HSPB1)單克隆抗體[ APPLICATIONS ]
  Western blotting: 0.5-5ug/ml
  Immunocytochemistry in formalin fixed cells: 5-30ug/ml
  Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
  Immunohistochemistry in paraffin section: 5-30ug/ml
  Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
  Optimal working dilutions must be determined by end user. [ FORMULATION ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant HSP27 disposed in loading
  buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
  Storage: Avoid repeated freeze/thaw cycles. Store at 4
  oC for frequent use. Aliquot and store at -20
  oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
  was determined by accelerated thermal degradation test, that is, incubate the
  protein at 37
  oC for 48h, and no obvious degradation and precipitation were
  observed.The loss rate is less than 5% within the expiration date under
  appropriate storage condition. 27kDa熱休克蛋白(HSPB1)單克隆抗體[ IDENTIFICATION ]
  A
  Figure 1. Western Blot
  A. Sample: Recombinant HSP27, Human B. Lane1: Human Lung Tissue Lane2: Porcine Muscle Tissue Lane3: Porcine Heart Tissue Primary Ab: μg/mL Rabbit Anti-Human HSP27 Ab
  Second Ab: 1:5000 Dilution of HRP-Linked Rabbit
  B Anti-Mouse IgG Ab (Catalog: SAA544Mu09)
  Figure 2. DAB staining on IHC-P Samples： A. Human Kidney Tissue B. Human Glioma Tissue C. Human Brain Tissue D. Human Stomach Tissue A B C D