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產品名稱: S100鈣結合蛋白(S100)多克隆抗體
產品型號: S100鈣結合蛋白多克隆抗體
產品展商: HZbscience
產品文檔: 無相關文檔
簡單介紹
S100鈣結合蛋白(S100)多克隆抗體是針對S100的兔多克隆抗體。在**組織化學染色和**印跡實驗中能識別S100。S100鈣結合蛋白(S100)多克隆抗體豐富產品組合，其中包括超過 42,000 多種高品質抗體，并配套提供種類齊全的抗體相關產品。 我們的抗體測定結果經全球上千次的引用驗證，有助于您在一系列抗體應用中獲得優異的實驗結果。
S100鈣結合蛋白(S100)多克隆抗體
的詳細介紹
S100鈣結合蛋白(S100)多克隆抗體
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來源多抗制備
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宿主兔
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效價-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標記物無標記物
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**原S100鈣結合蛋白(S100)多克隆抗體-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應用WB, ICC, IHC-P, IHC-F, ELISA
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S100鈣結合蛋白(S100)多克隆抗體Polyclonal Antibody to S100 Calcium Binding Protein (S100)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: S100, Bovine
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg Sample: Recombinant S100, Bovine
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant S100 (Met1~Ser94) expressed in E.coli. Accession No.: RPA012Bo01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MGSELETAME
TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK DADAVDKVMK ELDENGDGEV
DFQEYVVLVAALTVACNNFF WENS
[ ANTIBODY SPECIFITY ] S100鈣結合蛋白(S100)多克隆抗體The antibody is a rabbit polyclonal antibody raised against S100. It has been
selected for its ability to recognize S100 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant S100 (Met1~Ser94)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.S100鈣結合蛋白(S100)多克隆抗體
